Bone Healing in Ovariectomized-rabbit Calvarial Defect with Tricalcium Phosphate Coated with Recombinant Human Bone Morphogenetic Protein-2 Genetically Engineered in Escherichia coli
김중한, 김창주, 신상훈,
소속 상세정보
김중한 ( Kim Jung-Han ) - Pusan National University School of Dentistry Department of Oral and Maxillofacial Surgery
김창주 ( Kim Chang-Joo ) - Pusan National University School of Dentistry Department of Oral and Maxillofacial Surgery
신상훈 ( Shin Sang-Hun ) - Pusan National University School of Dentistry Department of Oral and Maxillofacial Surgery
KMID : 1036320140360020037
Abstract
Purpose: This study compares the bone formation ability of tricalcium phosphate (TCP) with and without recombinant human
bone morphogenetic protein-2 (rhBMP-2) and assesses TCP as a carrier of rhBMP-2.
Methods: Bilateral round defects (diameter: 8.0 mm) were formed in the cranium of eight New Zealand white rabbits.
The defects were grafted with TCP only (control group) or with rhBMP-2-coated TCP (experimental group). The animals
were sacrificed at 1st week, 2nd week, 4th week, and 8th week postoperatively; two rabbits sacrificed each time. The skulls
were harvested and subjected to radiographic and histological examination.
Results: Radiologic evaluation showed faster bone remodeling in the experimental group than in the control group. Histologic
evaluation (H&E, Masson’s trichrome stain) showed rapid bone formation, remodeling and calcification in the 1st and 2nd
week in the experimental group. Immunohistochemical evaluation showed higher expression rate of osteoprotegerin, receptor
activator of nuclear factor κB ligand, and receptor activator of nuclear factor κB in the experimental group at the 1st
and 2nd week than in the control group.
Conclusion: rhBMP-2 coated TCP resulted in rapid bone formation, remodeling, and calcification due to rhBMP-2’s osteogenic
effect. TCP performed properly as a carrier for rhBMP-2. Thus, the use of an rhBMP-2 coating on TCP had a synergic effect
on bone healing and, especially, bone remodeling and maturation.
키워드
Recombinant human bone morphogenetic protein-2; Tricalcium phosphate; Bone regeneration
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